Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis

Fig. 1

Panel a: The amino acid sequence of HPV-16 E7 containing SPusp45 signal peptide obtained from pNZ8123 vector was subjected to SignalP 4.1 analysis. SignalP graphical output represents the three different scores including raw cleavage site score (C-score) which is trained to distinguish signal peptide cleavage site; signal peptide score (S-score) which is trained to locate the signal peptides of a protein; and combination of C-score and the slope of S-score (Y- score). Furthermore, discrimination score (D-score) characterized the mean S and maximum Y scores, utilized for discriminating the signal peptide from its non-signal counterpart. Panel b: The protein tertiary structure of HPV-16 E7; the three-dimensional structure was established using SWISS-MODEL (http://swissmodel.expasy.org/). There were 13 α-helices and 10 β-sheets in the three-dimensional structure of HPV-16 E7. Panel c: Western blot analysis of rE7 proteins produced in L. lactis harboring pNZ8123-HPV16-optiE7 (right panel), prestained protein ladder (center panel), and pNZ8123-HPV16-E7 (left panel)

Back to article page
\