Fig. 3From: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1Positive feedback of Mxr1 increased the transcriptional efficiency of PAOX1 and broke the Mxr1 titration effect. mRNA was extracted from cells cultured in different carbon sources for 3 h. a GFP. b MXR1. mRNA levels in each sample were normalized to 18S rRNA. The relative expression level for each gene was normalized to the control grown under the carbon-free condition. The error bars represent the standard deviation of three biological replicates. Two-way ANOVA and the Tukey test were used to determine significance. Groups with different letters are significantly different. c AOX activity in different cells in response to different carbon sourcesBack to article page