Table 1 Primer sequences used in this study
From: pXST, a novel vector for TA cloning and blunt-end cloning
Name | Primer Sequences | Product size (bp) |
|---|---|---|
ccdB-F | CCAATACTTGTATGGgcagactggctgtgtataaggg | 487 |
ccdB-R | CCAATACTTGTATGGctccggtctggtaagcacaac | |
ccdBseq-F | TCTTTTGCTGACGAGAACAG | 210 + (insert) |
ccdBseq-R | CTTTCATCCCCGATATGCAC | |
M13F | GTAAAACGACGGCCAGT | 126 + (insert) |
M13R | CAGGAAACAGCTATGACC | |
F1 | GGCACAGACAAGATTGAACC | 517 |
R1 | AACACTGCAATTCCACGATG | |
F2 | TCCCGGTTGATTCTTTACGTCT | 957 |
R2 | CGCAGTTTCCCATTTGTAATCGTC | |
F3 | TCCCGGTTGATTCTTTACGTCT | 1515 |
R3 | CAATCTTGTCTGTGCCCCGAA | |
F4 | CTTTGCTTCCTATACCACGAGA | 2343 |
R4 | ACTAACACTGCAATTCCACGAT |