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Table 2 Analysis of the bidirectional DNA walking method, including the Cry-F and Cry-R walking direction, applied on GM events (2000 HGEs) containing the CryAb/c genes, using the four different mixes of DRT primers (A-D)

From: Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

 

Cry-F

Cry-R

Bt11 maize

+

+

 

A

B

C

D

A

B

C

D

 

550

500

650

950

1250

450

1000

1400*

1800

400*

800

> 2000

500

1000

1400

550

1300

1600

MON810 maize

+

+

A

B

C

D

A

B

C

D

550*

850

1300

650

 

400

250*

770

350

500

260

T304–40 cotton

+

+

A

B

C

D

A

B

C

D

550

800

350

450

500

600

800*

1200

400

600

550

200

200

450

550

200

450

700*

MON531 cotton

+

+

A

B

C

D

A

B

C

D

550

500*

950

450

1000

1700

1900

> 2000

350

600

1000

1400

 

750*

1400

 

MON87701 soybean

+

+

A

B

C

D

A

B

C

D

550

450

530

500

1000

> 2000

430

1000*

1700

> 2000

320

450

550

860

550

700

1000

1800*

550

660

700

1000

300

470

850

KeFeng-6 rice

+

+

A

B

C

D

A

B

C

D

550

440

520

720

970

250

470

950

790

430

980*

1650

700

1000

500

750

1100

370

500

800

1200

950*

1500

1800

MON15985 cotton

+

+

A

B

C

D

A

B

C

D

550

500

900*

430

800

1000

> 1500

350

600

1000

1500

> 2000

500

800*

1000

1200

220

281–24-236 × 3006–210-23 cotton

+

+

A

B

C

D

A

B

C

D

200

450

900

1000

 

550

420*

300

550

520

550

750*

 

MON89034 maize

+

+

A

B

C

D

A

B

C

D

600

850

1000*

660

 

420

500

650

900

300*

600

550

750

Bt176 maize

+

+

A

B

C

D

A

B

C

D

550

250

600

400*

550

670

1000

350

500

300

600

350

450*

500

850

1000

1700

  1. For each result, the experiment was carried out in triplicate. The detection of the targets is symbolized by + (amplicons observed in each repetition) or (+) (amplicons not observed in each repetition). The approximate size of amplicons is indicated in base-pair for each DRT primers. The amplicons observed in each repetition (3/3) are indicated in bold while the amplicons obtained not in each repetition (1–2/3) are indicated in italic. The sequenced amplicons are indicated by an asterisk