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Table 1 Oligonucleotide primers used for the real-time PCR assays, the DNA walking methods and the PCR confirmation of the Cry sequence from the T304–40 event

From: Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Methods

Oligonucleotide names

Oligonucleotide sequences

Product sizes (bp)

References

SYBR®Green qPCR

Cry1Ab/c-F

ACCGGTTACACTCCCATCGA

73

[13]

Cry1Ab/c-R

CAGCACCTGGCACGAACTC

[13]

DNA Walking

Cry-F a

TGCATTCCATACAACTGCTTGAG

 

This study

Cry-F b (Cry1Ab/c-F)

ACCGGTTACACTCCCATCGA

/

[13]

Cry-F c

GAGTTCGTGCCAGGTGCTG

 

[13]

DNA Walking

Cry-R a (Cry1Ab/c-R)

CAGCACCTGGCACGAACTC

 

[13]

Cry-R b

TCGATGGGAGTGTAACCGGT

/

[13]

Cry-R c

CTCAAGCAGTTGTATGGAATGCA

 

This study

PCR

T304–40-Cry-F

ACATGGATTATGGCCCACAT

787

This study

T304–40-Cry-R

GTTGGCAGCTTGCACATAGA

This study