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Fig. 4 | BMC Biotechnology

Fig. 4

From: DNA assembly with error correction on a droplet digital microfluidics platform

Fig. 4

The Mondrian™ microfluidic cartridge. a Image of the cartridge. b Diagram of the cartridge electrode paths. This diagram of the chip comes from a screenshot of an ADE software. There are 50 μL reservoirs highlighted in blue, 10 μL reservoirs highlighted in red. Orange reservoirs were used to hold waste. Green reservoirs were used to collect final products. The configuration of the chip allowed to perform 8 reactions in parallel. c Magnified image of one lane of the microfluidic cartridge where the reactions were performed. The area highlighted in yellow was used for error correction reaction. The area highlighted in purple was used for Gibson assembly and PCR annealing/extension. The area highlighted in red was used for DNA denaturation during PCR and error correction pretreatment

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