Inverse predictions from 5 sites for miR-208a-3p levels with 95% confidence intervals in biofluids from pooled control (open circles) and 24 h isoproterenol (closed circles) treatment groups. In (a), no LLOQ threshold was applied. In (b), LLOQ thresholds were applied based on logistic model standard error and on baseline noise in (c). In total, 50/104 measurements were above the LLOQ for the baseline noise model and 52/104 for both of the logistic models. The methods agree on the common 50 and the two logistic methods are in exact agreement.