Fig. 4

Sf-RVN cell Maverick 1 and 2 ATP and CAP are more similar to homologous proteins encoded by other Mavericks than to related viral proteins. a Amino acid alignment of the AAA+ ATPase domain regions of Sf Maverick 1 and 2 ATP, vertebrate Maverick ATP, cowpox virus A32 ATPase, and A. honmai entomopoxvirus ATPase. Conserved amino acid residues that constitute the ATPase Walker A and B motifs are indicated with a filled circle. b Unrooted tree showing the phylogenetic relationships among Sf Maverick and other insect and vertebrate Maverick ATP proteins, cowpox virus A32 ATPAse and A. honmai entomopoxvirus ATPase. c Amino acid alignment of the N-terminal regions containing the PLA2 domain of Sf Maverick 1 and 2 CAP, human parvovirus B19 capsid, B. mori densovirus 1 capsid, and A. honmai entomopoxvirus capsid-like protein. Conserved amino acids involved in catalysis are indicated with a filled circle, those involved in Ca2+ cofactor binding with an asterisk, and those that are otherwise conserved with an plus sign. d Unrooted tree showing the phylogenetic relationships among Sf Maverick and other insect Maverick CAP proteins, human parvovirus B19 capsid, B. mori densovirus 1 capsid, and A. honmai entomopoxvirus capsid-like protein. Abbreviations: Ah, the summer fruit tortix Adoxophyes honmai; At, the navel orangeworm Amyelois transistella; Bm, the silkworm Bombyx mori; Dr, the zebrafish Danio rerio; Ln, the black garden ant Lasius niger; Px, the diamondback moth Plutella xylostella; Sf, the fall armyworm Spodoptera frugiperda (this study); Tc, the flour beetle Tribolium castaneum