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Fig. 5 | BMC Biotechnology

Fig. 5

From: Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

Fig. 5

Daily changes of dose-response curves of ELuc bioluminescence from 3-methylcholanthrene-treated 3D spheroids. The culture medium containing 300 μM D-luciferin and 3-methylcholanthrene (5, 2.5, 1.3, 0.6, and 0.3 μM) was refreshed every 2 to 3 days. 3D cultures and bioluminescence measurement were performed as described in Methods and legend in Fig. 4. The dose-dependent change of bioluminescence intensity was expressed as the percentage of untreated control (set at 100%). Bioluminescence intensity at 50% was marked by a dotted red line. Italic digits in the figures indicate culture day after initial treatment. Sequential changes of IC50 values are shown in bottom panels. Error bars indicate standard deviations (n = 4).

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