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Fig. 4 | BMC Biotechnology

Fig. 4

From: Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

Fig. 4

Daily changes of dose-response curves of ELuc bioluminescence from acetaminophen-treated 3D spheroids. Primary hepatocytes were seeded in the 96-well format Cell-ableTM, in which feeder cells (3T3-Swiss albino) were pre-seeded 1 day before hepatocyte seeding, at the density of 4 × 104 cells/well. The culture medium containing 300 μM D-luciferin and acetaminophen (5, 2.5, 1.3, 0.6, and 0.3 mM) was refreshed every 2 to 3 days. Bioluminescence from the spheroids on the indicated culture day was measured nondestructively every day for 5 s in each well using a luminometer. The dose-dependent change of bioluminescence intensity was expressed as the percentage of untreated control (set at 100%). Bioluminescence intensity at 50% was marked by a dotted red line. Italic digits in the figures indicate culture day after initial treatment. Sequential changes of IC50 values are shown in bottom panels. Error bars indicate standard deviations (n = 4)

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