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Fig. 2 | BMC Biotechnology

Fig. 2

From: Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

Fig. 2

ELuc bioluminescence in mouse primary hepatocytes isolated from CAG-ELuc/MI-MAC Tc mice. Primary hepatocytes were seeded in collagen-coated 35-mm dishes and incubated for 1 day. a Single-cell bioluminescence imaging of ELuc-expressing hepatocytes. After 1 day of culture, the culture medium was replaced with DMEM supplemented with 10% FBS and 500 μM D-luciferin. Images were acquired using 3-min exposure time and a 4x objective lens without binning. Scale bar indicates 50 μm. b Emission spectrum of ELuc from the hepatocytes. After 1 day of culture, the culture medium was replaced with DMEM supplemented with 10% FBS and 200 μM D-luciferin. The spectrum was measured without destroying the cells for 1 min. c Stability of ELuc in the hepatocytes. After 1 day of culture, the culture medium was replaced with RM101 medium supplemented with 10% FBS, 200 μM D-luciferin, and 100 μM cycloheximide. Bioluminescence was recorded in real time for 1 min at 10-min intervals at 37 °C under 5% CO2 atmosphere. The maximum value was set at 100%. Error bars indicate standard deviations (n = 4)

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