Fig. 6

ERCC signal response as a function of relative abundance in each of the four pools on the LifeTech NGS platform. In Panel a, each line represents an individual ERCC control, where grey = titrated, black = 1-to-1, and red = outlier. Partially detected and undetected ERCC controls are included at the bottom to indicate their targeted relative abundance. In Panel b, the centroid of each ERCC control is plotted, where the red line corresponds to the linear fitted model, open circles = within 99 % CI, and red circles = outliers. In Panel c, the slope of each ERCC is plotted, where the open circles = monotonic ERCC controls (ρ = 1), grey squares = non-monotonic, and red = outliers. Numbers adjacent to outliers in Panels b and c correspond to the last three digits of the Control ID in Table 3. In Panel d, each ERCC control is represented on the Bland-Altman plot of Pool 12 vs Pool 14, with outliers coded as in Panels b and c above