Fig. 1

Modified Latin square pool design. Five subpools of ERCC controls, each containing a unique set of transcripts at different target relative abundance (see Table 1), were combined in varying proportions. Subpools A, B, C, D, and E are shaded, black, white, light grey, medium grey, and dark grey, respectively. Subpool A is present as a constant 10 % component of each pool, the modification. Subpools B-E are mixed using a Latin square of proportions 10, 15, 25, and 40 %