Fig. 5
From: A new high-throughput screening-compatible gap junctional intercellular communication assay
Application of this assay system to HOS cells. HOS-I− and HOS-YFPQL cells were mixed at a 2:1 ratio and plated on a 96-well plate. After 24 h of incubation, the medium was changed to C-solution containing vehicle or 25 μM CBX, followed by further incubation for 10 min before the 10-s I−-YFP assay. The percent YFP quenching at 10 s was plotted against for each well. Z’ factor was calculated as described in the Methods section and shown in the graph