Fig. 2

Serial dilution of primary FMD Asia 1 virus RNA in vitro was amplified to 3D fragment using real-time PCR and general RT-PCR amplifying VP1 fragment based on immunomagnetic to conjugate with sdAb. a FMD Asia 1 virus mixed with IMNBs at the concentration of 10⁻¹ to 10⁻⁶ TCID₅₀/mL; b FMD Asia 1 virus was diluted at the concentration of 10⁻¹ to 10⁻⁶ TCID₅₀/mL (c) lane N, negative control using sterile PBS as template; lane P, positive control of primary FMD Asia 1 virus (10⁰ TCID₅₀/mL) reacted with MNB; lanes 1–6, Asia 1 virus mixed with MNB at the concentration of 10⁻¹ to 10⁻⁶ TCID₅₀/mL, respectively; lane M, DL2000 DNA marker 2000, 1000, 750, 500, 250, 100 bp; (d) lane N, negative control using sterile PBS as template; lane P, positive control of primary FMD Asia 1 virus (10⁰ TCID₅₀/mL); lanes 1–6, FMD Asia 1 virus at the concentration of 10⁻¹ to 10⁻⁶ TCID₅₀/mL, respectively; lane M, DL2000