High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo

Table 1 Nucleotide sequences of oligonucleotides designed for assembly of IFN-CSP^*

Primers	Location	Nucleotide sequence (from 5′ end to 3′ end)
SR-1	1–29	GGAATTCCATATG TGTGATCTGCCTCAAA
AR-1	14–71	GTGCCAGGAGCATCAAGGTACGACGGCTACCCAGGCTGTGGGTTTGAGGCAGATCACA
SR-2	53–110	ACCTTGATGCTCCTGGCACAGATGCGTCGTATCTCTCTTTTCTCCTGCTTGAAGGACC
AR-2	92–149	GGTTGCCAAACTCCTCCTGTGGAAATCCAAAGTCATGACGGTCCTTCAAGCAGGAGAA
SR-3	131–188	CAGGAGGAGTTTGGCAACCAGTTCCAAAAGGCTGAAACCATCCCTGTCCTCCATGAGA
AR-3	170–227	AGTCCTTTGTGCTGAAGAGATTGAAGATCTGCTGGATCATCTCATGGAGGACAGGGAT
SR-4	209–266	CTCTTCAGCACAAAGGACTCATCTGCTGCTTGGGATGAGACCCTCCTTGACAAATTCT
AR-4	248–305	AGGCTTCCAGGTCATTCAGCTGCTGGTAGAGTTCAGTGTAGAATTTGTCAAGGAGGGT
SR-5	287–344	CTGAATGACCTGGAAGCCTGTGTGATTCAGGGGGTGGGGGTGACAGAGACTCCACTGA
AR-5	326–383	GGAAGTATTTACGCACAGCCAGAATGGAGTCCTCCTTCATCAGTGGAGTCTCTGTCAC
SR-6	365–422	GCTGTGCGTAAATACTTCCAACGTATCACTCTCTATCTGAAAGAGAAGAAATACAGCC
AR-6	404–461	AACGCATGATTTCTGCACGGACAACCTCCCAGGCACAAGGGCTGTATTTCTTCTCTTT
SR-7	443–500	CGTGCAGAAATCATGCGTTCTTTTTCTTTGTCAACAAACTTGCAAGAAAGTTTACGTA
AR-7	482–525	CGTAATTTCTCGTTGTCTTCCTTACTACGTAAACTTTCTTGCAA
SR-8	507–561	AAGACAACGAGAAATTACGTAAACCAAAACATAAAAAATTAAAGCAACCAGCGGA
AR-8	545–578	CCGCTCGAG ATTAACCATCCGCTGGTTGCTTTAA

^*Letters in italic denote the overlapped parts in IFN-CSP. Restriction sites are underlined. Bold type indicates the additional terminal codon ‘TAAT’

ISSN: 1472-6750