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Fig. 5 | BMC Biotechnology

Fig. 5

From: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo

Fig. 5

Effect of cell density on the expression level of IFN-CSP in E. coli BL21/pET21b-IFN-CSP. a: SDS-PAGE analyses of IFN-CSP expression at different growth stages. Lane M: Protein molecular weight marker; Lane 1: E. coli BL21/pET21b-IFN-CSP before induction; Lanes 2–8: E. coli BL21/pET21b-IFN-CSP was induced at OD600 value of 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 and 2.0, respectively. b: Percentage and concentration of IFN-CSP were calculated by the target bands in SDS-PAGE (A)

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BMC Biotechnology

ISSN: 1472-6750

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