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Table 2 Screening for Bm86, Bd86 and Ba86 expression in the culture supernatant of P. pastoris transformants.

From: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

Recombinant strain

Parental strain

Recombinant protein

Total protein concentration (mg·L-1)a

Recombinant protein concentration (mg·L-1)b

% of total proteinc

GS115Moz9-1

GS115

Bm86

66.5

3.0

4.5

GS115Moz9-2*

GS115

Bm86

65.5

3.3

5.0

GS115Moz9-3

GS115

Bm86

65.3

1.0

1.5

KM71HMoz9-1

KM71H

Bm86

66.3

1.0

1.5

KM71HMoz9-2

KM71H

Bm86

66.8

3.0

4.5

KM71HMoz9-3

KM71H

Bm86

64.8

1.5

2.3

GS115Dec8-1

GS115

Bd86

64.4

1.0

1.6

GS115Dec8-2

GS115

Bd86

66.4

1.5

2.3

GS115Dec8-3

GS115

Bd86

66.0

1.5

2.3

KM71HDec8-1*

KM71H

Bd86

66.0

2.0

3.0

KM71HDec8-2

KM71H

Bd86

63.4

1.5

2.4

KM71HDec8-3

KM71H

Bd86

63.5

1.0

1.6

X33pBaI 1

X33

Ba86

49.7

1.0

2.0

X33pBaI 2

X33

Ba86

45.5

1.0

2.2

X33pBaI 3*

X33

Ba86

45.4

6.0

13.2

X33pBaII 1

X33

Ba86

55.8

5.5

9.8

X33pBaII 2

X33

Ba86

48.3

5.0

10.4

X33pBaII 3

X33

Ba86

46.9

4.0

8.5

  1. The experiments were conducted twice with similar results.
  2. aDetermined using the Bradford method with BSA as standard [49].
  3. bDetermined by semi-quantitative analysis in dot-blots using a standard curve constructed with known amounts of recombinant Bm86 extracted from Gavac (Revetmex).
  4. cDetermined as the percent of recombinant protein in total preoteins.
  5. *Recombinant strains with highest Bm86, Bd86 and Ba86 concentration in the culture supernatant were selected for fermentation and protein production.