Figure 3

Generation of the MMV replica. (a) Cell replica. The liquid in the original MMV containing E. coli cells (harboring the GFP plasmid) in which the wells collectively resemble a cherry blossom pattern was transferred and returned, leaving a minute droplet in each replica MMV (upper rows #1 ~ 5). All these MMVs were added with culture media and incubated at 37°C for 21 h to regenerate cells (lower row). (b) DNA replica. The replica image is that of the 10th replication (PCR amplified), whereas the original MMV is shown just after the 10 rounds of replica generation operation. Both are stained with SYBR green I.