Table 5 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus NN-XS methods
From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling
65°C, 65 min | |||||||
|---|---|---|---|---|---|---|---|
Sample code | DNA (ng/μl) | DNA (ng) | 260/280 | 260/230 | 260/280 | 260/230 | Protocol |
NCS 4a | 17.5 | 734 | 1.98 | 0.96 | 1.84 | 0.98 | GQ |
NCS 4b | 17.3 | 833 | 1.72 | 0.84 | 1.65 | 0.78 | NN-XS |
NCS 5a | 17.6 | 741 | 1.82 | 1.05 | 1.81 | 1.07 | GQ |
NCS 5b | 12.8 | 614 | 1.61 | 1.02 | 1.73 | 1.07 | NN-XS |
NCS 6a | 8.2 | 328 | 2.02 | 0.81 | 1.79 | 0.76 | GQ |
NCS 6b | 15.7 | 756 | 1.70 | 0.89 | 1.70 | 0.86 | NN-XS |
NCS 4-10a | 22.2 | 956 | 1.95 | 1.21 | 2.02 | 1.19 | GQ |
NCS 4-10b | 13.5 | 660 | 1.84 | 1.10 | 1.85 | 1.08 | NN-XS |
NCS 4-11a | 19.1 | 822 | 1.60 | 0.85 | 1.71 | 0.88 | GQ |
NCS 4-11b | 12.6 | 618 | 1.60 | 1.00 | 1.58 | 1.11 | NN-XS |
NCS 4-12a | 10.4 | 447 | 1.54 | 0.74 | 1.76 | 0.74 | GQ |
NCS 4-12b | 13.1 | 657 | 1.55 | 0.76 | 1.70 | 0.74 | NN-XS |