From: DNA polymerase preference determines PCR priming efficiency
Top 1% (655 in 65,536) | 8 bp window motif analyses for several DNA polymerase experiments | |||||||
---|---|---|---|---|---|---|---|---|
QTT-A | QTT-AR | PGT-A | PGT-AR | QTT-A63 | QTT-A65 | QTT-A67 | QHH-B | |
(GC,CG) motif at 3’ end of primer | 88% | 89% | 90% | 90% | 90% | 91% | 88% | 25% |
(GC,CG) motif at 5’ end of 6 N portion of the primer | 8% | 6% | 8% | 11% | 12% | 10% | 8% | 89% |
(TC) motif at first 2 bp of runway | 3% | 4% | 3% | 3% | 4% | 4% | 4% | 19% |
(CC) motif at first 2 bp of runway | 28% | 26% | 28% | 30% | 23% | 23% | 25% | 30% |
(CGC,CCG,GCG, GGC,TGC,TCG) motif at 3’ end of primer | 87% | 88% | 88% | 89% | 90% | 90% | 87% | 22% |
(AGC,ACG) motif at 3’ end of primer | 1% | 1% | 1% | 1% | 1% | 1% | 1% | 4% |
(ACG) motif at 3’ end of primer | 1% | 1% | 1% | 1% | 1% | 1% | 1% | 2% |
(AGC) motif at 3’ end of primer | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2% |