From: DNA polymerase preference determines PCR priming efficiency
8 bp window sequence | Top 30 ranked QTT-A sequences: comparison of rank in other amplification experiments | |||||||
---|---|---|---|---|---|---|---|---|
QTT-A | QTT-AR | PGT-A | PGT-AR | QTT-A63 | QTT-A65 | QTT-A67 | QHH-B | |
GGGGGC GG | 1 | 3 | 2 | 3 | 1 | 1 | 1 | 4180 |
TTGGGC GG | 2 | 1 | 3 | 9 | 8 | 5 | 2 | 20382 |
GGGCCG GG | 3 | 12 | 5 | 2 | 5 | 3 | 3 | 606 |
TGGCCG GG | 4 | 5 | 4 | 4 | 6 | 6 | 6 | 3065 |
GGTCCG GG | 5 | 6 | 1 | 1 | 3 | 2 | 4 | 1666 |
GGGTGC GG | 6 | 9 | 6 | 13 | 16 | 12 | 9 | 2377 |
GTGGGC GG | 7 | 11 | 10 | 5 | 9 | 7 | 11 | 1464 |
TTGCCG GG | 8 | 8 | 8 | 7 | 22 | 21 | 20 | 13440 |
GTGCCG GG | 9 | 13 | 7 | 8 | 7 | 13 | 17 | 220 |
GGGGGC CG | 10 | 19 | 21 | 26 | 2 | 4 | 5 | 3950 |
TGGTGC GG | 11 | 18 | 9 | 14 | 37 | 28 | 19 | 8530 |
TTGGGC CG | 12 | 10 | 19 | 24 | 21 | 27 | 14 | 26404 |
TGCCCG GG | 13 | 14 | 13 | 6 | 13 | 15 | 21 | 3370 |
GGTCCG GC | 14 | 17 | 11 | 12 | 18 | 18 | 23 | 3802 |
GGGGGC CA | 15 | 31 | 24 | 29 | 12 | 14 | 15 | 8559 |
GGGCCG GC | 16 | 32 | 30 | 15 | 10 | 9 | 28 | 2255 |
TGGCCG GC | 17 | 15 | 15 | 16 | 26 | 23 | 22 | 5312 |
TTGTGC GG | 18 | 27 | 23 | 40 | 51 | 69 | 44 | 17564 |
TGTCCG GG | 19 | 21 | 12 | 11 | 32 | 33 | 33 | 9598 |
TTGGGC CA | 20 | 16 | 34 | 35 | 58 | 51 | 47 | 29598 |
GGTGGC GG | 21 | 22 | 16 | 17 | 19 | 22 | 24 | 1890 |
GGGGGC CC | 22 | 36 | 47 | 45 | 15 | 17 | 13 | 10961 |
TTTGGC GG | 23 | 33 | 32 | 22 | 57 | 71 | 39 | 24345 |
GGCCCG GG | 24 | 26 | 14 | 10 | 17 | 16 | 18 | 508 |
TGGGCG GG | 25 | 29 | 29 | 23 | 29 | 47 | 40 | 5616 |
GGGCGC GG | 26 | 87 | 33 | 38 | 25 | 34 | 42 | 1337 |
TTGCCG GC | 27 | 23 | 31 | 32 | 46 | 52 | 65 | 18324 |
TTGGGC GC | 28 | 24 | 51 | 57 | 92 | 62 | 60 | 25137 |
GGGGGC GC | 29 | 47 | 58 | 64 | 27 | 24 | 27 | 9051 |
GGGGGC TG | 30 | 35 | 55 | 80 | 4 | 8 | 10 | 8558 |