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Table 1 Primers and probes used in the present study

From: Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA

 

Target

Primer/probe

Sequence (5′ →3′)

Reference (GenBank Acc No.)

conv-RT-qPCR

hcnC

hcnC-F

CCTGCCCCAGTCGTTCTTT

DeCoste et al (2011)(DQ788990)

hcnC-R

TGCAACTGCGGATACATTGC

hcnC-FAM

FAM-ATTTCGCCTTGCAGTCC-MGBNFQ

phlD

phlD-F

CGGCGGACGGAAAATTC

DeCoste et al (2011)(DQ788986)

phlD-R

CCGACCGGGTTCCAAGTC

phlD-FAM

FAM-TGATGAACTGGTCCTGCAA-MGBNFQ

DNF-RT-qCPR

hcnC

hcnC-F

CCTGCCCCAGTCGTTCTTT

DeCoste et al (2011)(DQ788990)

MYT4

CAGCTTGGTAGAATCGATCAGCTAC

hcnC-FAM

FAM-ATTTCGCCTTGCAGTCC-MGBNFQ

HMYT4

CAGCTTGGTAGAATCGATCAGCTACTGCAACTGCGGATACATTGC

Present work

phlD

phlD-F

CGGCGGACGGAAAATTC

DeCoste et al (2011)(DQ788986)

MYT4

CAGCTTGGTAGAATCGATCAGCTAC

phlD-FAM

FAM-TGATGAACTGGTCCTGCAA-MGBNFQ

PMYT4

CAGCTTGGTAGAATCGATCAGCTACCCGACCGGGTTCCAAGTC

Present work

  1. : Reverse transcription primer. Underlined sequence is the binding site for the MYT4 primer.
  2. MGBNFQ: Minor groove-binding nonfluorescent quencher.