Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA

Figure 4

Conventional qRT-PCR assays of hcnC and phlD genes using non-treated (RT minus control assay) and DNase-treated RNA samples. DNase R1, R2 and R3 represent first, second and third round of DNase treatment of RNA extracted from liquid bacterial culture of Pseudomonas sp. LBUM300. Bars are standard error of the mean. PhlD-F/R and hcnC-F/R primer combination used for amplification of phlD and hcnC genes, respectively. An aliquot of the RT-qPCR reaction was run on 3% agarose gel to verify the amplification (bottom panel).

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us