Figure 7From: Less is more: strategies to remove marker genes from transgenic plantsZFN technology to modify an allele at a target locus. 1. Custom-designed ZFN is used to target the target allele ‘A’. 2. ZFN-mediated cleavage to induce a DSB at allele ‘A’. 3. The broken ends were repaired through endogenous repairing pathway. 4. If the broken ends are repaired through NHEJ pathway, a mutated allele (‘A*’) will be generated due to deletions or additions. 5. If HR repairing pathway is used and plasmids with a ‘replacement’ allele (A’, which has partial homologous sequences to allele A) are provided, the allele at the target locus can be replaced to A’.Back to article page