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Table 2 Data metrics from MiSeq sequencing of standard and modified Nextera library preparations

From: Improved workflows for high throughput library preparation using the transposome-based nextera system

 

Genomes trial

Reference genome

P. falciparum

C. difficile

E. coli

M. tuberculosis

% GC

19

29

50

66

Size (Mb)

23.3

4.3

5.2

4.4

Sample

Standard

Reaction E

Standard

Reaction E

Standard

Reaction E

Standard

Reaction E

Yield (Mb) Q20

432

260

278

457

171

220

184

194

% Mapped Reads

96.6

96.8

97.8

98.4

96.5

96.6

95.6

96.0

% Duplicates

0.5

0.5

0.8

1.6

0.4

0.7

0.5

0.6

% GC

22.7

22

28.6

30.4

48.4

48.6

61.8

61.8

  1. Genome trial data metrics. Samples were pooled and sequenced on a 150 bp PE Miseq Run. Data from the standard and the modified reaction were compared and showed no significant differences between the library preparations.