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Figure 2 | BMC Biotechnology

Figure 2

From: Improved workflows for high throughput library preparation using the transposome-based nextera system

Figure 2

Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A). Evaluation of the Axyprep Mag Normaliser kit (B): two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C): standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D): M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

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