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Table 2 Primers designed in this study and used for recombination

From: Engineering of the LukS-PV and LukF-PV subunits of Staphylococcus aureusPanton-Valentine leukocidin for Diagnostic and Therapeutic Applications

Primer Ida

Primer sequence in 5′ → 3′ directionb

Amplicon

(Size in bp)

rlukS-PV-1

GGC/CATGGATAACAATATTGAGAATATTGGTGATGG

rlukS-PV (868 bp)

rlukS-PV-2

GGCC/TCGAGATTATGTCCTTTCACTTTAATTTCATGAG

rlukF-PV-1

GGCC/CATGGCTCAACATATCACACCTGTAAG

rlukF-PV (919 bp)

rlukF-PV-2

GGCC/TCGAGGCTCATAGGATTTTTTCCTTAGATTGAG

  1. a Primer identification.
  2. b RDE sequences bold and underlined.
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BMC Biotechnology

ISSN: 1472-6750

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