Table 1 Bacterial isolates and plasmids used for genetic engineering

S. aureus MW2 (USA400)

PVL-positive CA-MRSA^a

Network on antimicrobial resistance in Staphylococcus aureus (NARSA)

pGEM^®-T Easy Vector

Intermediate plasmid vector

Lab stock maintained by Dr. Philip Bardelang [34]

Escherichia coli DH-5α

Intermediate E. coli host

pET-21d(+)

Protein expression plasmid vector

E. coli BL21(DE3)pLysS

Protein expression E. coli host